The study investigates the Leadership style of Institutions Leaders in Ghana.  It is also sought to determine if a statistically significant relationship existed between the Leadership styles of Institutions leaders and their Gender. Research on Institutions Leaders (formal leaders) in different settings suggests that Leadership style, managerial decision-making style, and Motivation are essential factors for managerial effectiveness (Bass, 1990). The study used the Leadership Questionnaire (Lewin, Lippit and White (1939) 18 questions to determine Leadership style. Thus, leadership styles are taken as the primary dependent variable, and Gender was taken as Independent variables for this study. A survey of 130 Institutions Leaders (51 males and 79 females) was selected from 179 Institutions in Ghana for this study. This research hypothesized that there is no statistically significant relationship between the dependent variable leadership Style and an independent variable, Gender. Hypotheses tested using Independent Sample T-Test. The finding reveals that most of the Institution's Leaders in Ghana are Democratic leaders. Therefore there is a statistically significant difference between the means of the two groups. Moreover, it also revealed a statistically significant relationship between the leadership style of Institutions Leaders and their Gender and found a significant negative relationship in leadership style between the Male and Female Leaders.